Quantitative and Qualitative Changes in V-J α Rearrangements During Mouse Thymocytes Differentiation

Quantitative and Qualitative Changes in V-J α Rearrangements During Mouse Thymocytes Differentiation

Knowledge of the complete nucleotide sequence of the mouse TCRAD locus allows an accurate determination V-J rearrangement status. Using multiplex genomic PCR assays and real time PCR analysis, we report a comprehensive and systematic analysis of the V-J recombination of TCR alpha chain in normal mouse thymocytes during development. These respective qualitative and quantitative approaches give r...

Qualitative and Quantitative Morphological Changes in Subcellular Fractions from Mouse Cerebral Cortex during Postnatal Development

Discontinuous Ficoll-sucrose gradients were used to prepare subcellular fractions from mouse cerebral cortex at various stages of postnatal development. Representative samples of each subcellular fraction were obtained by sedimentation in an analytical ultracentrifuge and each fraction was examined quantitatively and qualitatively by electron microscopy. The amount of synaptosomal material was ...

Concurrent V(D)J recombination and DNA end instability increase interchromosomal trans-rearrangements in ATM-deficient thymocytes

During the CD4(-)CD8(-) (DN) stage of T-cell development, RAG-dependent DNA breaks and V(D)J recombination occur at three T-cell receptor (TCR) loci: TCRβ, TCRγ and TCRδ. During this stage, abnormal trans-rearrangements also take place between TCR loci, occurring at increased frequency in absence of the DNA damage response mediator ataxia telangiectasia mutated (ATM). Here, we use this model of...

QUANTITATIVE AND QUALITATIVE CHANGES IN SHOOT APICAL MERISTEM OF SAFFRON CROCUS DURING THE PERIOD OF GROWTH AND DEVELOPMENT

Effects of Nitric Oxide on Qualitative and Qualitative Differentiation of Mouse Uterus

Purpose: We studied of quality and quantity differentation of mouse uterus after the injection of L-Name, L-Arg and normal saline on the 3rd, 4th and 5th days of pregnancy. Materials and Methods: Twenty mice that were 8 weeks old and 200-250g were kept in animal house conditions for one week. By looking at the vaginal plaque, we devided the pregnant mice into 4 groups of 5, consisting of: 1/co...